Ontology highlight
ABSTRACT:
SUBMITTER: Lauring J
PROVIDER: S-EPMC3042798 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Lauring J J Cosgrove D P DP Fontana S S Gustin J P JP Konishi H H Abukhdeir A M AM Garay J P JP Mohseni M M Wang G M GM Higgins M J MJ Gorkin D D Reis M M Vogelstein B B Polyak K K Cowherd M M Buckhaults P J PJ Park B H BH
Oncogene 20100125 16
An oncogenic mutation (G49A:E17K) in the AKT1 gene has been described recently in human breast, colon, and ovarian cancers. The low frequency of this mutation and perhaps other selective pressures have prevented the isolation of human cancer cell lines that harbor this mutation thereby limiting functional analysis. Here, we create a physiologic in vitro model to study the effects of this mutation by using somatic cell gene targeting using the nontumorigenic human breast epithelial cell line, MCF ...[more]