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ABSTRACT:
SUBMITTER: Sokolowska M
PROVIDER: S-EPMC3045582 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Sokolowska Monika M Czapinska Honorata H Bochtler Matthias M
Nucleic acids research 20101008 4
The GIY-YIG nuclease domain is present in all kingdoms of life and has diverse functions. It is found in the eukaryotic flap endonuclease and Holliday junction resolvase Slx1-Slx4, the prokaryotic nucleotide excision repair proteins UvrC and Cho, and in proteins of 'selfish' genetic elements. Here we present the structures of the ternary pre- and post-cleavage complexes of the type II GIY-YIG restriction endonuclease Hpy188I with DNA and a surrogate or catalytic metal ion, respectively. Our stru ...[more]