Ontology highlight
ABSTRACT:
SUBMITTER: Knutsen JH
PROVIDER: S-EPMC3046126 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
PloS one 20110228 2
The cell cycle of the fission yeast, Schizosaccharomyces pombe, does not easily lend itself to analysis by flow cytometry, mainly because cells in G(1) and G(2) phase contain the same amount of DNA. This occurs because fission yeast cells under standard growth conditions do not complete cytokinesis until after G(1) phase. We have devised a flow cytometric method exploiting the fact that cells in G(1) phase contain two nuclei, whereas cells in G(2) are mononuclear. Measurements of the width as we ...[more]