Proteome-wide systems analysis of a cellulosic biofuel-producing microbe.
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ABSTRACT:
SUBMITTER: Tolonen AC
PROVIDER: S-EPMC3049413 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Publications
Proteome-wide systems analysis of a cellulosic biofuel-producing microbe.
Tolonen Andrew C AC Haas Wilhelm W Chilaka Amanda C AC Aach John J Gygi Steven P SP Church George M GM
Molecular systems biology 20110101
Fermentation of plant biomass by microbes like Clostridium phytofermentans recycles carbon globally and can make biofuels from inedible feedstocks. We analyzed C. phytofermentans fermenting cellulosic substrates by integrating quantitative mass spectrometry of more than 2500 proteins with measurements of growth, enzyme activities, fermentation products, and electron microscopy. Absolute protein concentrations were estimated using Absolute Protein EXpression (APEX); relative changes between treat ...[more]
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