Poly(A) RT-PCR measurement of diagnostic genes in pancreatic juice in pancreatic cancer.

ABSTRACT:

Background

The last decade has seen significant progress in understanding the molecular biology of pancreatic adenocarcinoma. There is now an urgent need to translate these molecular techniques to clinical practice in order to improve diagnosis and prediction of response to treatment. The objectives of this study are to utilise poly(A) RT-PCR to measure expression levels of diagnostic Indicator genes, selected from microarray studies, of RNA from intraoperatively sampled pancreatic ductal juice and to correlate these expression levels with those in matched pancreatic tissue resection samples.

Methods

Intraoperative sampling of pancreatic juice and collection of matched tissue samples was undertaken in patients undergoing pancreaticoduodenectomy for suspected tumour. RNA was

SUBMITTER: Oliveira-Cunha M 

PROVIDER: S-EPMC3049557 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature