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Visualization of color anatomy and molecular fluorescence in whole-mouse cryo-imaging.

ABSTRACT: We developed multi-scale, live-time interactive visualization of color image data, including microscopic whole-mouse cryo-images serving many biomedical applications. Using true-color volume rendering, we interactively, selectively enhanced anatomy using feature detection. For example, to enhance red organs (vessels, liver, etc.) and internal surfaces, we computed a red feature from R/(R+G+B) and surface features from color/gray-scale gradients, respectively. For >70GB cryo-image volumes, we developed multi-resolution visualization, which provided low-resolution rendering of an entire mouse and zooming to organs, tissues, and cells. Fusions of fluorescence and color cryo-volumes uniquely showed biodistribution of metastatic and stem cells within an anatomical context.

SUBMITTER: Gargesha M

PROVIDER: S-EPMC3049862 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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Visualization of color anatomy and molecular fluorescence in whole-mouse cryo-imaging.

Gargesha Madhusudhana M Qutaish Mohammed Q MQ Roy Debashish D Steyer Grant J GJ Watanabe Michiko M Wilson David L DL

Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society 20101030 3

We developed multi-scale, live-time interactive visualization of color image data, including microscopic whole-mouse cryo-images serving many biomedical applications. Using true-color volume rendering, we interactively, selectively enhanced anatomy using feature detection. For example, to enhance red organs (vessels, liver, etc.) and internal surfaces, we computed a red feature from R/(R+G+B) and surface features from color/gray-scale gradients, respectively. For >70GB cryo-image volumes, we dev ...[more]

PMID: 21041065

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