Ontology highlight
ABSTRACT:
SUBMITTER: Kimzey MJ
PROVIDER: S-EPMC3050118 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Kimzey Michael J MJ Yassine Hussein N HN Riepel Brent M BM Tsaprailis George G Monks Terrence J TJ Lau Serrine S SS
Chemico-biological interactions 20101008 1-2
Methylglyoxal (MG) is a biologically reactive byproduct of glucose metabolism, levels of which increase in diabetes. MG modification of protein generates neutral hydroimidazolone adducts on arginine residues which can alter functional active sites. We investigated the site-specificity of MG adduction to human serum albumin (HSA) using multiple reaction monitoring (MRM) of 13 MG-modified tryptic peptides, each containing an internal arginine. Seven new sites for MG modification (R257>R209>R222>R8 ...[more]