Unknown

Dataset Information

0

Metformin inhibits P-glycoprotein expression via the NF-?B pathway and CRE transcriptional activity through AMPK activation.


ABSTRACT:

Background and purpose

The expression of P-glycoprotein (P-gp), encoded by the multidrug resistance 1 (MDR1) gene, is associated with the emergence of the MDR phenotype in cancer cells. We investigated whether metformin (1,1-dimethylbiguanide hydrochloride) down-regulates MDR1 expression in MCF-7/adriamycin (MCF-7/adr) cells.

Experimental approach

MCF-7 and MCF-7/adr cells were incubated with metformin and changes in P-gp expression were determined at the mRNA, protein and functional level. Transient transfection assays were performed to assess its gene promoter activities, and immunoblot analysis to study its molecular mechanisms of action.

Key results

Metformin significantly inhibited MDR1 expression by blocking MDR1 gene transcription. Metformin also significantly increased the intracellular accumulation of the fluorescent P-gp substrate rhodamine-123. Nuclear factor-?B (NF-?B) activity and the level of I?B degradation were reduced by metformin treatment. Moreover, transduction of MCF-7/adr cells with the p65 subunit of NF-?B induced MDR1 promoter activity and expression, and this effect was attenuated by metformin. The suppression of MDR1 promoter activity and protein expression was mediated through metformin-induced activation of AMP-activated protein kinase (AMPK). Small interfering RNA methods confirmed that reduction of AMPK levels attenuates the inhibition of MDR1 activation associated with metformin exposure. Furthermore, the inhibitory effects of metformin on MDR1 expression and cAMP-responsive element binding protein (CREB) phosphorylation were reversed by overexpression of a dominant-negative mutant of AMPK.

Conclusions and implications

These results suggest that metformin activates AMPK and suppresses MDR1 expression in MCF-7/adr cells by inhibiting the activation of NF-?B and CREB. This study reveals a novel function of metformin as an anticancer agent.

SUBMITTER: Kim HG 

PROVIDER: S-EPMC3051382 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Metformin inhibits P-glycoprotein expression via the NF-κB pathway and CRE transcriptional activity through AMPK activation.

Kim Hyung Gyun HG   Hien Tran Thi TT   Han Eun Hee EH   Hwang Yong Pil YP   Choi Jae Ho JH   Kang Keon Wook KW   Kwon Kwang-il KI   Kim Bong-Hee BH   Kim Sang Kyum SK   Song Gye Yong GY   Jeong Tae Cheon TC   Jeong Hye Gwang HG  

British journal of pharmacology 20110301 5


<h4>Background and purpose</h4>The expression of P-glycoprotein (P-gp), encoded by the multidrug resistance 1 (MDR1) gene, is associated with the emergence of the MDR phenotype in cancer cells. We investigated whether metformin (1,1-dimethylbiguanide hydrochloride) down-regulates MDR1 expression in MCF-7/adriamycin (MCF-7/adr) cells.<h4>Experimental approach</h4>MCF-7 and MCF-7/adr cells were incubated with metformin and changes in P-gp expression were determined at the mRNA, protein and functio  ...[more]

Similar Datasets

| S-EPMC6786375 | biostudies-literature
| S-EPMC6330460 | biostudies-literature
| S-EPMC8675877 | biostudies-literature
| S-EPMC7511631 | biostudies-literature
| S-EPMC9908748 | biostudies-literature
| S-EPMC10286367 | biostudies-literature
| S-EPMC4312923 | biostudies-literature
| S-EPMC7275116 | biostudies-literature
| S-EPMC5960613 | biostudies-literature
| S-EPMC3613409 | biostudies-literature