Efficient microwave-assisted synthesis of human islet amyloid polypeptide designed to facilitate the specific incorporation of labeled amino acids.
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ABSTRACT:
SUBMITTER: Marek P
PROVIDER: S-EPMC3052696 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Publications
Efficient microwave-assisted synthesis of human islet amyloid polypeptide designed to facilitate the specific incorporation of labeled amino acids.
Marek Peter P Woys Ann Marie AM Sutton Kelvin K Zanni Martin T MT Raleigh Daniel P DP
Organic letters 20101101 21
A cost-efficient, time-reducing solid-phase synthesis of the amyloidogenic, 37 residue islet amyloid polypeptide (IAPP) is developed using two pseudoprolines (highlighted blue in sequence) in combination with microwave technology. A yield twice that obtained with conventional syntheses is realized. The utility of this protocol is demonstrated by the synthesis of a (13)C(18)O-labeled Ser-20 IAPP variant, a prohibitively expensive and chemically challenging site to label via other protocols. TEM a ...[more]
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