Ontology highlight
ABSTRACT:
SUBMITTER: Xu F
PROVIDER: S-EPMC3052767 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Xu Fei F Wang Wei-Han WH Tan Yu-Jing YJ Bruening Merlin L ML
Analytical chemistry 20101118 24
Sequential adsorption of poly(styrene sulfonate) and trypsin in nylon membranes provides a simple, inexpensive method to create stable, microporous reactors for fast protein digestion. The high local trypsin concentration and short radial diffusion distances in membrane pores facilitate proteolysis in residence times of a few seconds, and the minimal pressure drop across the thin membranes allows their use in syringe filters. Membrane digestion and subsequent MS analysis of bovine serum albumin ...[more]