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Fluorescence detection of single-nucleotide polymorphisms with a single, self-complementary, triple-stem DNA probe.

ABSTRACT: Singled out for its singularity: In a single-step, single-component, fluorescence-based method for the detection of single-nucleotide polymorphisms at room temperature, the sensor is comprised of a single, self-complementary DNA strand that forms a triple-stem structure. The large conformational change that occurs upon binding to perfectly matched (PM) targets results in a significant increase in fluorescence (see picture; F = fluorophore, Q = quencher).

SUBMITTER: Xiao Y

PROVIDER: S-EPMC3053031 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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Fluorescence detection of single-nucleotide polymorphisms with a single, self-complementary, triple-stem DNA probe.

Xiao Yi Y Plakos Kory J I KJ Lou Xinhui X White Ryan J RJ Qian Jiangrong J Plaxco Kevin W KW Soh H Tom HT

Angewandte Chemie (International ed. in English) 20090101 24

Singled out for its singularity: In a single-step, single-component, fluorescence-based method for the detection of single-nucleotide polymorphisms at room temperature, the sensor is comprised of a single, self-complementary DNA strand that forms a triple-stem structure. The large conformational change that occurs upon binding to perfectly matched (PM) targets results in a significant increase in fluorescence (see picture; F = fluorophore, Q = quencher). ...[more]

PMID: 19431180

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