Ontology highlight
ABSTRACT:
SUBMITTER: Russell JD
PROVIDER: S-EPMC3056920 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Russell Jason D JD Hilger Ryan T RT Ladror Daniel T DT Tervo Mark A MA Scalf Mark M Shortreed Michael R MR Coon Joshua J JJ Smith Lloyd M LM
Analytical chemistry 20110211 6
Direct mass spectrometric quantification of peptides and proteins is compromised by the wide variabilities in ionization efficiency which are hallmarks of both the MALDI and ESI ionization techniques. We describe here the implementation of a fluorescence detection system for measurement of the UV-excited intrinsic fluorescence (UV-IF) from peptides and proteins just prior to their exit and electrospray ionization from an ESI capillary. The fluorescence signal provides a quantifiable measure of t ...[more]