Ontology highlight
ABSTRACT:
SUBMITTER: Jiang X
PROVIDER: S-EPMC3057939 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Jiang Xiaoxu X Nie Yiling Y Kaback H Ronald HR
Biochemistry 20110208 10
In total, 59 single Cys-replacement mutants in helix VII and helix X of the lactose permease of Escherichia coli were subjected to site-directed fluorescence labeling in right-side-out membrane vesicles to complete the testing of Cys accessibility or reactivity. For both helices, accessibility/reactivity is relatively low at the level of the sugar-binding site where the helices are tightly packed. However, labeling of Cys substitutions in helix VII with tetramethylrhodamine-5-maleimide decreases ...[more]