Exploring the oral microbiota of children at various developmental stages of their dentition in the relation to their oral health.

ABSTRACT:

Background

An understanding of the relation of commensal microbiota to health is essential in preventing disease. Here we studied the oral microbial composition of children (N = 74, aged 3 - 18 years) in natural transition from their deciduous to a permanent dentition and related the microbial profiles to their oral health status. The microbial composition of saliva was assessed by barcoded pyrosequencing of the V5-V6 hypervariable regions of the 16 S rRNA, as well as by using phylogenetic microarrays.

Results

Pyrosequencing reads (126174 reads, 1045 unique sequences) represented 8 phyla and 113 higher taxa in saliva samples. Four phyla--Firmicutes, Bacteriodetes, Proteobacteria and Actinobacteria--predominated in all groups. The deciduous dentition harboured a higher propor

SUBMITTER: Crielaard W 

PROVIDER: S-EPMC3058002 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature