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The cellular receptor to human rhinovirus 2 binds around the 5-fold axis and not in the canyon: a structural view.

ABSTRACT: Human rhinovirus serotype 2 (HRV2) belongs to the minor group of HRVs that bind to members of the LDL-receptor family including the very low density lipoprotein (VLDL)-receptor (VLDL-R). We have determined the structures of the complex between HRV2 and soluble fragments of the VLDL-R to 15 A resolution by cryo-electron microscopy. The receptor fragments, which include the first three ligand-binding repeats of the VLDL-R (V1-3), bind to the small star-shaped dome on the icosahedral 5-fold axis. This is in sharp contrast to the major group of HRVs where the receptor site for ICAM-1 is located at the base of a depression around each 5-fold axis. Homology models of the three domains of V1-3 were used to explore the virus-receptor interaction. The footprint of VLDL-R on the viral surface covers the BC- and HI-loops on VP1.

SUBMITTER: Hewat EA

PROVIDER: S-EPMC305862 | biostudies-literature | 2000 Dec

REPOSITORIES: biostudies-literature

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The cellular receptor to human rhinovirus 2 binds around the 5-fold axis and not in the canyon: a structural view.

Hewat E A EA Neumann E E Conway J F JF Moser R R Ronacher B B Marlovits T C TC Blaas D D

The EMBO journal 20001201 23

Human rhinovirus serotype 2 (HRV2) belongs to the minor group of HRVs that bind to members of the LDL-receptor family including the very low density lipoprotein (VLDL)-receptor (VLDL-R). We have determined the structures of the complex between HRV2 and soluble fragments of the VLDL-R to 15 A resolution by cryo-electron microscopy. The receptor fragments, which include the first three ligand-binding repeats of the VLDL-R (V1-3), bind to the small star-shaped dome on the icosahedral 5-fold axis. T ...[more]

PMID: 11101504

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