Ontology highlight
ABSTRACT:
SUBMITTER: Liu S
PROVIDER: S-EPMC3058889 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Nature structural & molecular biology 20101121 12
Human immunodeficiency virus (HIV) initiates reverse transcription of its viral RNA (vRNA) genome from a cellular tRNA(3)(Lys) primer. This process is characterized by a slow initiation phase with specific pauses, followed by a fast elongation phase. We report a single-molecule study that monitors the dynamics of individual initiation complexes, comprised of vRNA, tRNA and HIV reverse transcriptase (RT). RT transitions between two opposite binding orientations on tRNA-vRNA complexes, and the pro ...[more]