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Rapid blue-light-mediated induction of protein interactions in living cells.

ABSTRACT: Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light exposure with subsecond time resolution and subcellular spatial resolution. We demonstrate the utility of this system by inducing protein translocation, transcription and Cre recombinase-mediated DNA recombination using light.

SUBMITTER: Kennedy MJ

PROVIDER: S-EPMC3059133 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

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Rapid blue-light-mediated induction of protein interactions in living cells.

Kennedy Matthew J MJ Hughes Robert M RM Peteya Leslie A LA Schwartz Joel W JW Ehlers Michael D MD Tucker Chandra L CL

Nature methods 20101031 12

Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light exposure with subsecond time resolution and subcellular spatial resolution. We demonstrate the utility of this system by inducing protein translocation, transcription and Cre r ...[more]

PMID: 21037589

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