HSSB1 rapidly binds at the sites of DNA double-strand breaks and is required for the efficient recruitment of the MRN complex.
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ABSTRACT:
SUBMITTER: Richard DJ
PROVIDER: S-EPMC3061066 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Publications
hSSB1 rapidly binds at the sites of DNA double-strand breaks and is required for the efficient recruitment of the MRN complex.
Richard Derek J DJ Savage Kienan K Bolderson Emma E Cubeddu Liza L So Sairei S Ghita Mihaela M Chen David J DJ White Malcolm F MF Richard Kerry K Prise Kevin M KM Schettino Giuseppe G Khanna Kum Kum KK
Nucleic acids research 20101103 5
hSSB1 is a newly discovered single-stranded DNA (ssDNA)-binding protein that is essential for efficient DNA double-strand break signalling through ATM. However, the mechanism by which hSSB1 functions to allow efficient signalling is unknown. Here, we show that hSSB1 is recruited rapidly to sites of double-strand DNA breaks (DSBs) in all interphase cells (G1, S and G2) independently of, CtIP, MDC1 and the MRN complex (Rad50, Mre11, NBS1). However expansion of hSSB1 from the DSB site requires the ...[more]
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