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RNAi screening for fat regulatory genes with SRS microscopy.

ABSTRACT: Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genetic regulators of fat storage.

SUBMITTER: Wang MC

PROVIDER: S-EPMC3061290 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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RNAi screening for fat regulatory genes with SRS microscopy.

Wang Meng C MC Min Wei W Freudiger Christian W CW Ruvkun Gary G Xie X Sunney XS

Nature methods 20110116 2

Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genet ...[more]

PMID: 21240281

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