Ontology highlight
ABSTRACT:
SUBMITTER: Tonelli M
PROVIDER: S-EPMC3061545 | biostudies-literature | 2009 Oct
REPOSITORIES: biostudies-literature
Tonelli Marco M Masterson Larry R LR Cornilescu Gabriel G Markley John L JL Veglia Gianluigi G
Journal of the American Chemical Society 20091001 40
We present a procedure that supports the acquisition of (1)H-(15)N residual dipolar coupling (RDC) values for individual subunits in binary or ternary protein assemblies from a single experimental sample. Our method relies on asymmetric labeling of each subunit with the following scheme: species A uniformly with (15)N, species B uniformly with (15)N and (13)C, and species C uniformly with (15)N but selectively with (13)C' or (13)C(alpha). Because only a single sample is required, the approach ob ...[more]