Accurate genome-scale percentage DNA methylation estimates from microarray data.
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ABSTRACT:
SUBMITTER: Aryee MJ
PROVIDER: S-EPMC3062148 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Publications
Accurate genome-scale percentage DNA methylation estimates from microarray data.
Aryee Martin J MJ Wu Zhijin Z Ladd-Acosta Christine C Herb Brian B Feinberg Andrew P AP Yegnasubramanian Srinivasan S Irizarry Rafael A RA
Biostatistics (Oxford, England) 20100921 2
DNA methylation is a key regulator of gene function in a multitude of both normal and abnormal biological processes, but tools to elucidate its roles on a genome-wide scale are still in their infancy. Methylation sensitive restriction enzymes and microarrays provide a potential high-throughput, low-cost platform to allow methylation profiling. However, accurate absolute methylation estimates have been elusive due to systematic errors and unwanted variability. Previous microarray preprocessing pr ...[more]
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