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IKK? phosphorylation regulates RPS3 nuclear translocation and NF-?B function during infection with Escherichia coli strain O157:H7.

ABSTRACT: NF-?B is a major gene regulator in immune responses, and ribosomal protein S3 (RPS3) is an NF-?B subunit that directs specific gene transcription. However, it is unknown how nuclear translocation of RPS3 is regulated. Here we report that phosphorylation of RPS3 Ser209 by the kinase IKK? was crucial for nuclear localization of RPS3 in response to activating stimuli. Moreover, virulence protein NleH1 of the foodborne pathogen Escherichia coli strain O157:H7 specifically inhibited phosphorylation of RPS3 Ser209 and blocked RPS3 function, thereby promoting bacterial colonization and diarrhea but resulting in less mortality in a gnotobiotic piglet-infection model. Thus, the IKK?-dependent modification of a specific amino acid in RPS3 promoted specific NF-?B functions that underlie the molecular pathogenetic mechanisms of E. coli O157:H7.

SUBMITTER: Wan F

PROVIDER: S-EPMC3062687 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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IKKβ phosphorylation regulates RPS3 nuclear translocation and NF-κB function during infection with Escherichia coli strain O157:H7.

Wan Fengyi F Weaver Amanda A Gao Xiaofei X Bern Michael M Hardwidge Philip R PR Lenardo Michael J MJ

Nature immunology 20110313 4

NF-κB is a major gene regulator in immune responses, and ribosomal protein S3 (RPS3) is an NF-κB subunit that directs specific gene transcription. However, it is unknown how nuclear translocation of RPS3 is regulated. Here we report that phosphorylation of RPS3 Ser209 by the kinase IKKβ was crucial for nuclear localization of RPS3 in response to activating stimuli. Moreover, virulence protein NleH1 of the foodborne pathogen Escherichia coli strain O157:H7 specifically inhibited phosphorylation o ...[more]

PMID: 21399639

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