Ontology highlight
ABSTRACT:
SUBMITTER: Verma R
PROVIDER: S-EPMC3063307 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Verma Rati R Oania Robert R Fang Ruihua R Smith Geoffrey T GT Deshaies Raymond J RJ
Molecular cell 20110101 1
Cdc48/p97 is an essential ATPase whose role in targeting substrates to the ubiquitin-proteasome system (UPS) remains unclear. Existing models posit that Cdc48 acts upstream of UPS receptors. To address this hypothesis, we examined the association of ubiquitin (Ub) conjugates with 26S proteasomes. Unexpectedly, proteasomes isolated from cdc48 mutants contain high levels of Ub conjugates, and mass spectrometry identified numerous nonproteasomal proteins, including Rpb1, the largest subunit of RNA ...[more]