Dataset Information

MicroRNA expression signatures of bladder cancer revealed by deep sequencing.

ABSTRACT:

Background

MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression. They are aberrantly expressed in many types of cancers. In this study, we determined the genome-wide miRNA profiles in bladder urothelial carcinoma by deep sequencing.

Methodology/principal findings

We detected 656 differentially expressed known human miRNAs and miRNA antisense sequences (miRNA*s) in nine bladder urothelial carcinoma patients by deep sequencing. Many miRNAs and miRNA*s were significantly upregulated or downregulated in bladder urothelial carcinoma compared to matched histologically normal urothelium. hsa-miR-96 was the most significantly upregulated miRNA and hsa-miR-490-5p was the most significantly downregulated one. Upregulated miRNAs were more common than downregulated ones. The hsa-miR-183, hsa-miR-200b ? 429, hsa-miR-200c ? 141 and hsa-miR-17 ? 92 clusters were significantly upregulated. The hsa-miR-143 ? 145 cluster was significantly downregulated. hsa-miR-182, hsa-miR-183, hsa-miR-200a, hsa-miR-143 and hsa-miR-195 were evaluated by Real-Time qPCR in a total of fifty-one bladder urothelial carcinoma patients. They were aberrantly expressed in bladder urothelial carcinoma compared to matched histologically normal urothelium (p < 0.001 for each miRNA).

Conclusions/significance

To date, this is the first study to determine genome-wide miRNA expression patterns in human bladder urothelial carcinoma by deep sequencing. We found that a collection of miRNAs were aberrantly expressed in bladder urothelial carcinoma compared to matched histologically normal urothelium, suggesting that they might play roles as oncogenes or tumor suppressors in the development and/or progression of this cancer. Our data provide novel insights into cancer biology.

SUBMITTER: Han Y

PROVIDER: S-EPMC3065473 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

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Publications

MicroRNA expression signatures of bladder cancer revealed by deep sequencing.

Han Yonghua Y Chen Jiahao J Zhao Xiaokun X Liang Chaozhao C Wang Yong Y Sun Liang L Jiang Zhimao Z Zhang Zhongfu Z Yang Ruilin R Chen Jing J Li Zesong Z Tang Aifa A Li Xianxin X Ye Jiongxian J Guan Zhichen Z Gui Yaoting Y Cai Zhiming Z

PloS one 20110328 3

<h4>Background</h4>MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression. They are aberrantly expressed in many types of cancers. In this study, we determined the genome-wide miRNA profiles in bladder urothelial carcinoma by deep sequencing.<h4>Methodology/principal findings</h4>We detected 656 differentially expressed known human miRNAs and miRNA antisense sequences (miRNA*s) in nine bladder urothelial carcinoma patients by deep sequencing. Many miRNAs and miRNA*s ...[more]

PMID: 21464941

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Dataset Information

MicroRNA expression signatures of bladder cancer revealed by deep sequencing.

Background

Methodology/principal findings

Conclusions/significance

Publications

MicroRNA expression signatures of bladder cancer revealed by deep sequencing.

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