Ontology highlight
ABSTRACT:
SUBMITTER: Schmidt S
PROVIDER: S-EPMC3067276 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Schmidt Sabine S Rainieri Sandra S Witte Simone S Matern Ulrich U Martens Stefan S
Applied and environmental microbiology 20110107 5
Baker's yeast (Saccharomyces cerevisiae) whole-cell bioconversions of naringenin 7-O-β-glucoside revealed considerable β-glucosidase activity, which impairs any strategy to generate or modify flavonoid glucosides in yeast transformants. Up to 10 putative glycoside hydrolases annotated in the S. cerevisiae genome database were overexpressed with His tags in yeast cells. Examination of these recombinant, partially purified polypeptides for hydrolytic activity with synthetic chromogenic α- or β-glu ...[more]