Proof of concept for recombinant cellular controls in quantitative molecular pathogen detection.
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ABSTRACT:
SUBMITTER: Rossmanith P
PROVIDER: S-EPMC3067449 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Publications
Proof of concept for recombinant cellular controls in quantitative molecular pathogen detection.
Rossmanith Peter P Mester Patrick P Frühwirth Karin K Fuchs Sabine S Wagner Martin M
Applied and environmental microbiology 20110211 7
In this study, we present the concept of internal sample process controls (ISPCs) to monitor the efficiency of an analytical chain using sample preparation and quantitative PCR (qPCR). A recombinant Listeria monocytogenes ΔprfA (targeted deletion) strain containing a competitive artificial single-copy genomic target was applied to naturally contaminated samples to demonstrate its analytical suitability as an ISPC. ...[more]
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