Shuttle vector system for Methanococcus maripaludis with improved transformation efficiency.
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ABSTRACT:
SUBMITTER: Walters AD
PROVIDER: S-EPMC3067461 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Publications
Shuttle vector system for Methanococcus maripaludis with improved transformation efficiency.
Walters Alison D AD Smith Sarah E SE Chong James P J JP
Applied and environmental microbiology 20110204 7
We have identified an open reading frame and DNA element that are sufficient to maintain shuttle vectors in Methanococcus maripaludis. Strain S0001, containing ORF1 from pURB500 integrated into the M. maripaludis genome, supports a significantly smaller shuttle vector, pAW42, and a 7,000-fold increase in transformation efficiency for pURB500-based vectors. ...[more]
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