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Global identification of multiple substrates for Plasmodium falciparum SUB1, an essential malarial processing protease.


ABSTRACT: The protozoan pathogen responsible for the most severe form of human malaria, Plasmodium falciparum, replicates asexually in erythrocytes within a membrane-bound parasitophorous vacuole (PV). Following each round of intracellular growth, the PV membrane (PVM) and host cell membrane rupture to release infectious merozoites in a protease-dependent process called egress. Previous work has shown that, just prior to egress, an essential, subtilisin-like parasite protease called PfSUB1 is discharged into the PV lumen, where it directly cleaves a number of important merozoite surface and PV proteins. These include the essential merozoite surface protein complex MSP1/6/7 and members of a family of papain-like putative proteases called SERA (serine-rich antigen) that are implicated in egress. To determine whether PfSUB1 has additional, previously unrecognized substrates, we have performed a bioinformatic and proteomic analysis of the entire late asexual blood stage proteome of the parasite. Our results demonstrate that PfSUB1 is responsible for the proteolytic processing of a range of merozoite, PV, and PVM proteins, including the rhoptry protein RAP1 (rhoptry-associated protein 1) and the merozoite surface protein MSRP2 (MSP7-related protein-2). Our findings imply multiple roles for PfSUB1 in the parasite life cycle, further supporting the case for considering the protease as a potential new antimalarial drug target.

SUBMITTER: Silmon de Monerri NC 

PROVIDER: S-EPMC3067499 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

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Global identification of multiple substrates for Plasmodium falciparum SUB1, an essential malarial processing protease.

Silmon de Monerri Natalie C NC   Flynn Helen R HR   Campos Marta G MG   Hackett Fiona F   Koussis Konstantinos K   Withers-Martinez Chrislaine C   Skehel J Mark JM   Blackman Michael J MJ  

Infection and immunity 20110110 3


The protozoan pathogen responsible for the most severe form of human malaria, Plasmodium falciparum, replicates asexually in erythrocytes within a membrane-bound parasitophorous vacuole (PV). Following each round of intracellular growth, the PV membrane (PVM) and host cell membrane rupture to release infectious merozoites in a protease-dependent process called egress. Previous work has shown that, just prior to egress, an essential, subtilisin-like parasite protease called PfSUB1 is discharged i  ...[more]

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