Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle.
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ABSTRACT:
SUBMITTER: Eckert SE
PROVIDER: S-EPMC3067669 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
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Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle.
Eckert Sabine E SE Dziva Francis F Chaudhuri Roy R RR Langridge Gemma C GC Turner Daniel J DJ Pickard Derek J DJ Maskell Duncan J DJ Thomson Nicholas R NR Stevens Mark P MP
Journal of bacteriology 20110128 7
Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use. ...[more]
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