Ontology highlight
ABSTRACT:
SUBMITTER: Kuo HC
PROVIDER: S-EPMC3067732 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Kuo Hsiao-Che HC Wang Ting-Yu TY Chen Peng-Peng PP Chen Young-Mao YM Chuang Hui-Ching HC Chen Tzong-Yueh TY
Journal of clinical microbiology 20110112 3
Viral nervous necrosis caused by nervous necrosis virus (NNV) exacts a high mortality and results in huge economic losses in grouper aquaculture in Taiwan. The present study developed a real-time quantitative PCR (qPCR) method for NNV monitoring. The assay showed a strong linear correlation (r(2) = 0.99) between threshold cycle (C(T)) and RNA quantities, which allowed identification of infected groupers by the C(T) value and could be exploited to warn of NNV infection prior to an outbreak in gro ...[more]