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Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay.

ABSTRACT: A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.

SUBMITTER: Choi GE

PROVIDER: S-EPMC3067748 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

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Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay.

Choi Go-Eun GE Chang Chulhun L CL Whang Jake J Kim Hwa-Jung HJ Kwon O Jung OJ Koh Won-Jung WJ Shin Sung Jae SJ

Journal of clinical microbiology 20101222 3

A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species. ...[more]

PMID: 21177894

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