Ontology highlight
ABSTRACT:
SUBMITTER: Iliuk A
PROVIDER: S-EPMC3069141 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Iliuk Anton A Martinez Juan S JS Hall Mark C MC Tao W Andy WA
Analytical chemistry 20110311 7
Quantitative phosphorylation analysis is essential to understanding cellular signal transductions. Here we present a novel technology for the highly efficient assay of protein phosphorylation in high-throughput format without the use of phospho-specific antibodies. The technique is based on a water-soluble, nanosize polymer, termed pIMAGO, that is multifunctionalized with titanium(IV) ions for specific binding to phosphoproteins and with biotin groups that allow for enzyme-linked spectrometric d ...[more]