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An infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides.


ABSTRACT: Lactating mothers secrete milk sialyloligosaccharides (MSOs) that function as anti-adhesives once provided to the neonate. Particular infant-associated commensals, such as Bifidobacterium longum subsp. infantis, consume neutral milk oligosaccharides, although their ability to utilize acidic oligosaccharides has not been assessed. Temporal glycoprofiling of acidic HMO consumed during fermentation demonstrated a single composition, with several isomers, corresponding to sialylated lacto-N-tetraose. To utilize MSO, B. longum subsp. infantis deploys a sialidase that cleaves ?2-6 and ?2-3 linkages. NanH2, encoded within the HMO catabolic cluster is up-regulated during HMO fermentation and is active on sialylated lacto-N-tetraose. These results demonstrate that commensal microorganisms do utilize MSO, a substrate that may be enriched in the distal gastrointestinal tract.

SUBMITTER: Sela DA 

PROVIDER: S-EPMC3069393 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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An infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides.

Sela David A DA   Li Yanhong Y   Lerno Larry L   Wu Shuai S   Marcobal Angela M AM   German J Bruce JB   Chen Xi X   Lebrilla Carlito B CB   Mills David A DA  

The Journal of biological chemistry 20110202 14


Lactating mothers secrete milk sialyloligosaccharides (MSOs) that function as anti-adhesives once provided to the neonate. Particular infant-associated commensals, such as Bifidobacterium longum subsp. infantis, consume neutral milk oligosaccharides, although their ability to utilize acidic oligosaccharides has not been assessed. Temporal glycoprofiling of acidic HMO consumed during fermentation demonstrated a single composition, with several isomers, corresponding to sialylated lacto-N-tetraose  ...[more]

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