Vaccine candidate P6 of nontypable Haemophilus influenzae is not a transmembrane protein based on protein structural analysis.
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ABSTRACT:
SUBMITTER: Michel LV
PROVIDER: S-EPMC3070289 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
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Vaccine candidate P6 of nontypable Haemophilus influenzae is not a transmembrane protein based on protein structural analysis.
Michel Lea Vacca LV Kalmeta Breanna B McCreary Mark M Snyder Joy J Craig Paul P Pichichero Michael E ME
Vaccine 20110105 8
P6 has been a vaccine candidate for nontypable Haemophilus influenzae (NTHi) based on its location on the outer membrane and immunogenicity. Because P6 is attached to the inner peptidoglycan layer of NTHi, and is putatively surface exposed, it must be a transmembrane protein. We examined the P6 structure using computational modeling, site-directed mutagenesis, and nuclear magnetic resonance spectroscopy. We found that P6 cannot be a transmembrane protein, and therefore may not be surface exposed ...[more]
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