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Divergent mechanisms for passive pneumococcal resistance to ?-lactam antibiotics in the presence of Haemophilus influenzae.


ABSTRACT: Otitis media, for which antibiotic treatment failure is increasingly common, is a leading pediatric public health problem.In vitro and in vivo studies using the chinchilla model of otitis media were performed using a ?-lactamase-producing strain of nontypeable Haemophilus influenzae (NTHi 86-028NP) and an isogenic mutant deficient in ?-lactamase production (NTHi 86-028NP bla) to define the roles of biofilm formation and ?-lactamase production in antibiotic resistance. Coinfection studies were done with Streptococcus pneumoniae to determine if NTHi provides passive protection by means of ?-lactamase production, biofilm formation, or both.NTHi 86-028NP bla was resistant to amoxicillin killing in biofilm studies in vitro; however, it was cleared by amoxicillin treatment in vivo, whereas NTHi 86-028NP was unaffected in either system. NTHi 86-028NP protected pneumococcus in vivo in both the effusion fluid and bullar homogenate. NTHi 86-028NP bla and pneumococcus were both recovered from the surface-associated bacteria of amoxicillin-treated animals; only NTHi 86-028NP bla was recovered from effusion.Based on these studies, we conclude that NTHi provides passive protection for S. pneumoniae in vivo through 2 distinct mechanisms: production of ?-lactamase and formation of biofilm communities.

SUBMITTER: Weimer KE 

PROVIDER: S-EPMC3071235 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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Divergent mechanisms for passive pneumococcal resistance to β-lactam antibiotics in the presence of Haemophilus influenzae.

Weimer Kristin E D KE   Juneau Richard A RA   Murrah Kyle A KA   Pang Bing B   Armbruster Chelsie E CE   Richardson Stephen H SH   Swords W Edward WE  

The Journal of infectious diseases 20110110 4


<h4>Background</h4>Otitis media, for which antibiotic treatment failure is increasingly common, is a leading pediatric public health problem.<h4>Methods</h4>In vitro and in vivo studies using the chinchilla model of otitis media were performed using a β-lactamase-producing strain of nontypeable Haemophilus influenzae (NTHi 86-028NP) and an isogenic mutant deficient in β-lactamase production (NTHi 86-028NP bla) to define the roles of biofilm formation and β-lactamase production in antibiotic resi  ...[more]

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