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Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells.

ABSTRACT: Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.

SUBMITTER: Munkley J

PROVIDER: S-EPMC3074132 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells.

Munkley Jennifer J Copeland Nikki A NA Moignard Victoria V Knight John R P JR Greaves Erin E Ramsbottom Simon A SA Pownall Mary E ME Southgate Jennifer J Ainscough Justin F-X JF Coverley Dawn D

Nucleic acids research 20101124 7

Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-R ...[more]

PMID: 21109536

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Aberrant expression of nuclear matrix proteins during HMBA-induced differentiation of gastric cancer cells.

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Altered profiles of nuclear matrix proteins during the differentiation of human gastric mucous adenocarcinoma MGc80-3 cells.

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