Ontology highlight
ABSTRACT:
SUBMITTER: Moretti R
PROVIDER: S-EPMC3075670 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Moretti Rocco R Chang Aram A Peltier-Pain Pauline P Bingman Craig A CA Phillips George N GN Thorson Jon S JS
The Journal of biological chemistry 20110211 15
Directed evolution is a valuable technique to improve enzyme activity in the absence of a priori structural knowledge, which can be typically enhanced via structure-guided strategies. In this study, a combination of both whole-gene error-prone polymerase chain reaction and site-saturation mutagenesis enabled the rapid identification of mutations that improved RmlA activity toward non-native substrates. These mutations have been shown to improve activities over 10-fold for several targeted substr ...[more]