Dataset Information

Structural and immunologic cross-reactivity among filarial and mite tropomyosin: implications for the hygiene hypothesis.

ABSTRACT:

Background

The hygiene hypothesis suggests that parasitic infection modulates host immune responses and decreases atopy. Other data suggest parasitic infections may induce allergic responsiveness.

Objective

To assess the structural and immunologic relationships between the known Dermatophagoides pteronyssinus (Der p 10) tropomyosin allergen and filarial tropomyosin of Onchocerca volvulus (OvTrop).

Methods

The molecular, structural, and immunologic relationships between OvTrop and Der p 10 were compared. Levels of OvTrop-specific and Der p 10-specific IgE, IgG, and IgG? in sera of filaria-infected and filarial-uninfected D pteronyssinus-atopic individuals were compared, as were the responses in nonhuman primates infected with the filarial parasite Loa loa. Cross-reactivity was compared by antigen-mediated depletion assays and functionality by passive basophil sensitization.

Results

Filarial and mite tropomyosins were very similar, with 72% identity at the amino acid level, and overlapping predicted 3-dimensional structures. The prevalence of IgE and IgG to Der p 10 was increased in filaria-infected individuals compared with uninfected subjects. There was a strong correlation between serum levels of Ov- and Der p 10-tropomyosin-specific IgE, IgG, and IgG? (P < .0001; r > 0.79). Preincubation of sera from anti-Der p 10-positive subjects with OvTrop completely depleted IgE, IgG, and IgG? anti-Der p 10. Basophils sensitized with sera from individuals allergic to Der p 10 released histamine similarly when triggered with OvTrop or Der p 10. Primates experimentally infected with L loa developed IgE that cross-reacted with Der p 10.

Conclusion

Filarial infection induces strong cross-reactive antitropomyosin antibody responses that may affect sensitization and regulation of allergic reactivity.

SUBMITTER: Santiago HC

PROVIDER: S-EPMC3075728 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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Publications

Structural and immunologic cross-reactivity among filarial and mite tropomyosin: implications for the hygiene hypothesis.

Santiago Helton C HC Bennuru Sasisekhar S Boyd Alexis A Eberhard Mark M Nutman Thomas B TB

The Journal of allergy and clinical immunology 20101224 2

<h4>Background</h4>The hygiene hypothesis suggests that parasitic infection modulates host immune responses and decreases atopy. Other data suggest parasitic infections may induce allergic responsiveness.<h4>Objective</h4>To assess the structural and immunologic relationships between the known Dermatophagoides pteronyssinus (Der p 10) tropomyosin allergen and filarial tropomyosin of Onchocerca volvulus (OvTrop).<h4>Methods</h4>The molecular, structural, and immunologic relationships between OvTr ...[more]

PMID: 21185070

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Dataset Information

Structural and immunologic cross-reactivity among filarial and mite tropomyosin: implications for the hygiene hypothesis.

Background

Objective

Methods

Results

Conclusion

Publications

Structural and immunologic cross-reactivity among filarial and mite tropomyosin: implications for the hygiene hypothesis.

Similar Datasets

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