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A novel auxiliary subunit critical to BK channel function in Caenorhabditis elegans.


ABSTRACT: The BK channel is a Ca²+- and voltage-gated potassium channel with many important physiological functions. To identify proteins important to its function in vivo, we screened for Caenorhabditis elegans mutants that suppressed a lethargic phenotype caused by expressing a gain-of-function (gf) isoform of the BK channel ?-subunit SLO-1. BKIP-1 (for BK channel interacting protein), a small peptide with no significant homology to any previously characterized molecules, was thus identified. BKIP-1 and SLO-1 showed similar expression and subcellular localization patterns and appeared to interact physically through discrete domains. bkip-1 loss-of-function (lf) mutants phenocopied slo-1(lf) mutants in behavior and synaptic transmission and suppressed the lethargy, egg-laying defect, and deficient neurotransmitter release caused by SLO-1(gf). In heterologous expression systems, BKIP-1 decreased the activation rate and shifted the conductance-voltage relationship of SLO-1 in a Ca²+-dependent manner and increased SLO-1 surface expression. Thus, BKIP-1 is a novel auxiliary subunit critical to SLO-1 function in vivo.

SUBMITTER: Chen B 

PROVIDER: S-EPMC3076056 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

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A novel auxiliary subunit critical to BK channel function in Caenorhabditis elegans.

Chen Bojun B   Ge Qian Q   Xia Xiao-Ming XM   Liu Ping P   Wang Sijie J SJ   Zhan Haiying H   Eipper Betty A BA   Wang Zhao-Wen ZW  

The Journal of neuroscience : the official journal of the Society for Neuroscience 20101201 49


The BK channel is a Ca²+- and voltage-gated potassium channel with many important physiological functions. To identify proteins important to its function in vivo, we screened for Caenorhabditis elegans mutants that suppressed a lethargic phenotype caused by expressing a gain-of-function (gf) isoform of the BK channel α-subunit SLO-1. BKIP-1 (for BK channel interacting protein), a small peptide with no significant homology to any previously characterized molecules, was thus identified. BKIP-1 and  ...[more]

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