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Hepatitis C virus NS2 protein triggers endoplasmic reticulum stress and suppresses its own viral replication.

ABSTRACT: We previously reported that the NS2 protein of hepatitis C virus (HCV) inhibits the expression of reporter genes driven by a variety of cellular and viral promoters. The aim of the study was to determine whether the broad transcriptional repression is caused by endoplasmic reticulum (ER) stress.Phosphorylation of the translation initiation factor eIF2? and HCV replication was detected by Western and Northern blot, respectively. De novo protein synthesis was measured by metabolic labeling. Activation of ER stress responsive genes was determined by promoter reporter assay, as well as mRNA and protein measurement by real time PCR and Western blot.Transient or inducible NS2 protein expression increased eIF2? phosphorylation and reduced de novo protein synthesis. It up-regulated promoter activities and transcript levels of ER stress inducible genes including GRP78, ATF6, and GADD153, as well as GRP78 protein level. The same effect was observed when NS2 was synthesized as part of the core-E1-E2-p7-NS2 polypeptide. NS2 protein also inhibited reporter gene expression from the HCV internal ribosome entry site and consequently reduced HCV replication. The full-length HCV replicon activated GRP78, ATF6, and GADD153 promoters more efficiently than the subgenomic replicon lacking the coding sequence for both the structural proteins and NS2. Abrogation of HCV infection/replication, by an inhibitor of the NS3 protease, relieved ER stress.HCV infection can induce ER stress, with NS2 protein being a major mediator. The stress can be relieved by a feedback mechanism.

SUBMITTER: von dem Bussche A

PROVIDER: S-EPMC3077220 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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Hepatitis C virus NS2 protein triggers endoplasmic reticulum stress and suppresses its own viral replication.

von dem Bussche Annette A Machida Raiki R Li Ke K Loevinsohn Gideon G Khander Amrin A Wang Jianguo J Wakita Takaji T Wands Jack R JR Li Jisu J

Journal of hepatology 20100724 5

<h4>Background & aims</h4>We previously reported that the NS2 protein of hepatitis C virus (HCV) inhibits the expression of reporter genes driven by a variety of cellular and viral promoters. The aim of the study was to determine whether the broad transcriptional repression is caused by endoplasmic reticulum (ER) stress.<h4>Methods</h4>Phosphorylation of the translation initiation factor eIF2α and HCV replication was detected by Western and Northern blot, respectively. De novo protein synthesis ...[more]

PMID: 20801537

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Hepatitis C virus NS2 protein triggers endoplasmic reticulum stress and suppresses its own viral replication.

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Hepatitis C virus NS2 protein triggers endoplasmic reticulum stress and suppresses its own viral replication.

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