Ontology highlight
ABSTRACT:
SUBMITTER: Aronson DE
PROVIDER: S-EPMC3079558 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Aronson Deborah E DE Costantini Lindsey M LM Snapp Erik L EL
Traffic (Copenhagen, Denmark) 20110225 5
The ability to study proteins in live cells using genetically encoded fluorescent proteins (FPs) has revolutionized cell biology (1-3). Researchers have created numerous FP biosensors and optimized FPs for specific organisms and subcellular environments in a rainbow of colors (4,5). However, expressing FPs in oxidizing environments such as the eukaryotic endoplasmic reticulum (ER) or the bacterial periplasm can impair folding, thereby preventing fluorescence (6,7). A substantial fraction of enha ...[more]