Expression, purification, crystallization and preliminary X-ray analysis of wild-type and of an active-site mutant of glyceraldehyde-3-phosphate dehydrogenase from Campylobacter jejuni.

ABSTRACT: The genome of the enteric pathogen Campylobacter jejuni encodes a single glyceraldehyde-3-phosphate dehydrogenase that can utilize either NADP+ or NAD+ as coenzymes for the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate. Here, the cloning, expression, purification, crystallization and preliminary X-ray analysis of both the wild type and an active-site mutant of the enzyme are presented. Preliminary X-ray analysis revealed that in both cases the crystals diffracted to beyond 1.9?Å resolution. The space group is shown to be I4(1)22, with unit-cell parameters a=90.75, b=90.75, c=225.48?Å, ?=90.46, ?=90.46, ?=222.79°; each asymmetric unit contains only one subunit of the tetrameric enzyme.

SUBMITTER: Tourigny DS 

PROVIDER: S-EPMC3079976 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature