Optimizing non-natural protein function with directed evolution.
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ABSTRACT: Developing technologies such as unnatural amino acid mutagenesis, non-natural cofactor engineering, and computational design are generating proteins with novel functions; these proteins, however, often do not reach performance targets and would benefit from further optimization. Evolutionary methods can complement these approaches: recent work combining unnatural amino acid mutagenesis and phage selection has created useful proteins of novel composition. Weak initial activity in a computationally designed enzyme has been improved by iterative rounds of mutagenesis and screening. A marriage of ingenuity and evolution will expand the scope of protein function well beyond Mother Nature's designs.
SUBMITTER: Brustad EM
PROVIDER: S-EPMC3080047 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
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