N-terminal domain of alphaB-crystallin provides a conformational switch for multimerization and structural heterogeneity.

ABSTRACT: The small heat shock protein (sHSP) ?B-crystallin (?B) plays a key role in the cellular protection system against stress. For decades, high-resolution structural studies on heterogeneous sHSPs have been confounded by the polydisperse nature of ?B oligomers. We present an atomic-level model of full-length ?B as a symmetric 24-subunit multimer based on solid-state NMR, small-angle X-ray scattering (SAXS), and EM data. The model builds on our recently reported structure of the homodimeric ?-crystallin domain (ACD) and C-terminal IXI motif in the context of the multimer. A hierarchy of interactions contributes to build multimers of varying sizes: Interactions between two ACDs define a dimer, three dimers connected by their C-terminal regions define a hexameric unit, and variable interactions involving the N-terminal region define higher-order multimers. Within a multimer, N-terminal regions exist in multiple environments, contributing to the heterogeneity observed by NMR. Analysis of SAXS data allows determination of a heterogeneity parameter for this type of system. A mechanism of multimerization into higher-order asymmetric oligomers via the addition of up to six dimeric units to a 24-mer is proposed. The proposed asymmetric multimers explain the homogeneous appearance of ?B in negative-stain EM images and the known dynamic exchange of ?B subunits. The model of ?B provides a structural basis for understanding known disease-associated missense mutations and makes predictions concerning substrate binding and the reported fibrilogenesis of ?B.

SUBMITTER: Jehle S 

PROVIDER: S-EPMC3081008 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature