Gli1 enhances migration and invasion via up-regulation of MMP-11 and promotes metastasis in ERα negative breast cancer cell lines.
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ABSTRACT:
SUBMITTER: Kwon YJ
PROVIDER: S-EPMC3081062 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:PurposeThe function of curcumin on the gastric cancer cell line, SGC-7901 is unknown. The present study aimed to observe the effects of curcumin on gastric cancer cells through the Shh and Wnt signaling pathways.MethodsSGC-7901 cells were transfected with si-Gli1 and si-?-catenin siRNA, then cells were stimulated with curcumin and its effects on cell migration, invasion, cytoskeleton remodeling, EMT, apoptosis and cell cycle were investigated by ?transwell assays, immunofluorescence and flow cytometry assays. The interaction between Gli1 and ?-catenin was observed by co-immunoprecipitation.ResultsWe show that curcumin suppressed the expression of Shh, Gli1 and Foxm1 in the Shh signaling pathway, and the expression of ?-catenin in the Wnt signaling pathway in SGC-7901 cells, both in mRNA and protein. As a result, cellular migration, invasion and cytoskeletal remodeling ability decreased. Our results revealed that when stimulated with curcumin, cells showed decreased cellular migration and invasion, while enhanced apoptosis. In addition, curcumin induced cytoskeletal remodeling and S phase cell cycle arrest. The inhibition of Shh and Wnt signaling pathway and the addition of curcumin also inhibited the epithelial–mesenchymal transition process. Furthermore, a physical interaction was observed between Gli1 of the Shh signaling and ?-catenin of the Wnt signaling in these cells, but curcumin inhibited the interaction of these two proteins.ConclusionThe present study indicated that curcumin plays an anti-tumor role through Gli1-?-catenin pathway in gastric cancer SGC-7901 cells.
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