Ontology highlight
ABSTRACT:
SUBMITTER: Tikhonova EB
PROVIDER: S-EPMC3082741 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Tikhonova Elena B EB Yamada Yoichi Y Zgurskaya Helen I HI
Chemistry & biology 20110401 4
Multidrug efflux pumps adversely affect both the clinical effectiveness of existing antibiotics and the discovery process to find new ones. In this study, we reconstituted and characterized by surface plasmon resonance the assembly of AcrAB-TolC, the archetypal multidrug efflux pump from Escherichia coli. We report that the periplasmic AcrA and the outer membrane channel TolC assemble high-affinity complexes with AcrB transporter independently from each other. Antibiotic novobiocin and MC-207,11 ...[more]