Unknown

Dataset Information

0

Sequential mechanism of assembly of multidrug efflux pump AcrAB-TolC.


ABSTRACT: Multidrug efflux pumps adversely affect both the clinical effectiveness of existing antibiotics and the discovery process to find new ones. In this study, we reconstituted and characterized by surface plasmon resonance the assembly of AcrAB-TolC, the archetypal multidrug efflux pump from Escherichia coli. We report that the periplasmic AcrA and the outer membrane channel TolC assemble high-affinity complexes with AcrB transporter independently from each other. Antibiotic novobiocin and MC-207,110 inhibitor bind to the immobilized AcrB but do not affect interactions between components of the complex. In contrast, DARPin inhibits interactions between AcrA and AcrB. Mutational opening of TolC channel decreases stability of interactions and promotes disassembly of the complex. The conformation of the membrane proximal domain of AcrA is critical for the formation of AcrAB-TolC and could be targeted for the development of new inhibitors.

SUBMITTER: Tikhonova EB 

PROVIDER: S-EPMC3082741 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Sequential mechanism of assembly of multidrug efflux pump AcrAB-TolC.

Tikhonova Elena B EB   Yamada Yoichi Y   Zgurskaya Helen I HI  

Chemistry & biology 20110401 4


Multidrug efflux pumps adversely affect both the clinical effectiveness of existing antibiotics and the discovery process to find new ones. In this study, we reconstituted and characterized by surface plasmon resonance the assembly of AcrAB-TolC, the archetypal multidrug efflux pump from Escherichia coli. We report that the periplasmic AcrA and the outer membrane channel TolC assemble high-affinity complexes with AcrB transporter independently from each other. Antibiotic novobiocin and MC-207,11  ...[more]

Similar Datasets

| S-EPMC5404916 | biostudies-literature
| S-EPMC4361902 | biostudies-literature
| S-EPMC6570770 | biostudies-literature
| S-EPMC4332038 | biostudies-literature
| S-EPMC5553321 | biostudies-literature
| S-EPMC127391 | biostudies-literature
| S-EPMC6977953 | biostudies-literature
| S-EPMC3984920 | biostudies-literature
| S-EPMC10315565 | biostudies-literature
| S-EPMC4403819 | biostudies-literature