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Genome-wide evidence for an essential role of the human Staf/ZNF143 transcription factor in bidirectional transcription.

ABSTRACT: In the human genome, ? 10% of the genes are arranged head to head so that their transcription start sites reside within <1 kbp on opposite strands. In this configuration, a bidirectional promoter generally drives expression of the two genes. How bidirectional expression is performed from these particular promoters constitutes a puzzling question. Here, by a combination of in silico and biochemical approaches, we demonstrate that hStaf/ZNF143 is involved in controlling expression from a subset of divergent gene pairs. The binding sites for hStaf/ZNF143 (SBS) are overrepresented in bidirectional versus unidirectional promoters. Chromatin immunoprecipitation assays with a significant set of bidirectional promoters containing putative SBS revealed that 93% of them are associated with hStaf/ZNF143. Expression of dual reporter genes directed by bidirectional promoters are dependent on the SBS integrity and requires hStaf/ZNF143. Furthermore, in some cases, functional SBS are located in bidirectional promoters of gene pairs encoding a noncoding RNA and a protein gene. Remarkably, hStaf/ZNF143 per se exhibits an inherently bidirectional transcription activity, and together our data provide the demonstration that hStaf/ZNF143 is indeed a transcription factor controlling the expression of divergent protein-protein and protein-non-coding RNA gene pairs.

SUBMITTER: Anno YN

PROVIDER: S-EPMC3082894 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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Genome-wide evidence for an essential role of the human Staf/ZNF143 transcription factor in bidirectional transcription.

Anno Yannick-Noël YN Myslinski Evelyne E Ngondo-Mbongo Richard Patryk RP Krol Alain A Poch Olivier O Lecompte Odile O Carbon Philippe P

Nucleic acids research 20101221 8

In the human genome, ∼ 10% of the genes are arranged head to head so that their transcription start sites reside within <1 kbp on opposite strands. In this configuration, a bidirectional promoter generally drives expression of the two genes. How bidirectional expression is performed from these particular promoters constitutes a puzzling question. Here, by a combination of in silico and biochemical approaches, we demonstrate that hStaf/ZNF143 is involved in controlling expression from a subset of ...[more]

PMID: 21177654

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Genome-wide evidence for an essential role of the human Staf/ZNF143 transcription factor in bidirectional transcription.

Publications

Genome-wide evidence for an essential role of the human Staf/ZNF143 transcription factor in bidirectional transcription.

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