Ontology highlight
ABSTRACT:
SUBMITTER: Westhofen P
PROVIDER: S-EPMC3083210 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Westhofen Philipp P Watzka Matthias M Marinova Milka M Hass Moritz M Kirfel Gregor G Müller Jens J Bevans Carville G CG Müller Clemens R CR Oldenburg Johannes J
The Journal of biological chemistry 20110302 17
Human vitamin K 2,3-epoxide reductase complex subunit 1-like 1 (VKORC1L1), expressed in HEK 293T cells and localized exclusively to membranes of the endoplasmic reticulum, was found to support both vitamin K 2,3-epoxide reductase (VKOR) and vitamin K reductase enzymatic activities. Michaelis-Menten kinetic parameters for dithiothreitol-driven VKOR activity were: K(m) (μM) = 4.15 (vitamin K(1) epoxide) and 11.24 (vitamin K(2) epoxide); V(max) (nmol·mg(-1)·hr(-1)) = 2.57 (vitamin K(1) epoxide) and ...[more]