Fatty acid production in genetically modified cyanobacteria.
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ABSTRACT:
SUBMITTER: Liu X
PROVIDER: S-EPMC3084101 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Publications
Fatty acid production in genetically modified cyanobacteria.
Liu Xinyao X Sheng Jie J Curtiss Roy R
Proceedings of the National Academy of Sciences of the United States of America 20110411 17
To avoid costly biomass recovery in photosynthetic microbial biofuel production, we genetically modified cyanobacteria to produce and secrete fatty acids. Starting with introducing an acyl-acyl carrier protein thioesterase gene, we made six successive generations of genetic modifications of cyanobacterium Synechocystis sp. PCC6803 wild type (SD100). The fatty acid secretion yield was increased to 197 ± 14 mg/L of culture in one improved strain at a cell density of 1.0 × 10(9) cells/mL by adding ...[more]
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