Dataset Information

Unique uterine localization and regulation may differentiate LPA3 from other lysophospholipid receptors for its role in embryo implantation.

ABSTRACT:

Objective

To determine factors differentiating LPA3 from other lysophospholipid (LP) receptors for its role in embryo implantation.

Design

Experimental mouse models.

Setting

Institute/university research laboratories.

Animal(s)

Wild-type, Lpar3(-/-), Lpar1(-/-)Lpar2(-/-), and S1pr2(-/-)S1pr3(-/-) mice.

Intervention(s)

Ovariectomy.

Main outcome measure(s)

Blue dye injection for determining implantation sites on gestation day 4.5. Real-time polymerase chain reaction for measuring gene expression in whole uterus and separated uterine layers. In situ hybridization for detecting progesterone (P)-induced Lpar3 expression in the uterine luminal epithelium (LE).

Result(s)

Normal implantation was observed in Lpar1(-/-)Lpar2(-/-) and S1pr2(-/-)S1pr3(-/-) females. Temporal expression showed peak expression of Lpar3 in the preimplantation uterus and constitutive expression of the other nine LP receptors in the periimplantation uterus. Spatial localization revealed main expression of Lpar3 in the LE and broad expression of the remaining LP receptors in all three main uterine layers: LE, stromal, and myometrial layers. Hormonal regulation in ovariectomized uterus indicated up-regulation of Lpar3 but down-regulation or no effect of the remaining nine LP receptors by P, and down-regulation of most LP receptors, including Lpar3, by 17?-estradiol.

Conclusion(s)

LE localization and up-regulation by P differentiate LPA3 from the other nine LP receptors and may underlie its essential role in embryo implantation.

SUBMITTER: Ye X

PROVIDER: S-EPMC3084612 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Unique uterine localization and regulation may differentiate LPA3 from other lysophospholipid receptors for its role in embryo implantation.

Ye Xiaoqin X Herr Deron R DR Diao Honglu H Rivera Richard R Chun Jerold J

Fertility and sterility 20110315 6

<h4>Objective</h4>To determine factors differentiating LPA3 from other lysophospholipid (LP) receptors for its role in embryo implantation.<h4>Design</h4>Experimental mouse models.<h4>Setting</h4>Institute/university research laboratories.<h4>Animal(s)</h4>Wild-type, Lpar3(-/-), Lpar1(-/-)Lpar2(-/-), and S1pr2(-/-)S1pr3(-/-) mice.<h4>Intervention(s)</h4>Ovariectomy.<h4>Main outcome measure(s)</h4>Blue dye injection for determining implantation sites on gestation day 4.5. Real-time polymerase cha ...[more]

PMID: 21411082

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Project description:Myometrium plays critical roles in multiple processes such as embryo spacing through peristalsis during mouse implantation, indicating vital roles of smooth muscle in the successful establishment and quality of implantation. Actin, a key element of cytoskeleton structure, plays an important role in the movement and contraction of smooth muscle cells (SMCs). However, the function of peri-implantation uterine smooth muscle and the regulation mechanism of muscle tension are still unclear. This study focused on the molecular mechanism of actin assembly regulation on implantation in smooth muscle. Phalloidin is a highly selective bicyclic peptide used for staining actin filaments (also known as F-actin). Phalloidin staining showed that F-actin gradually weakened in the CD-1 mouse myometrium from day 1 to day 4 of early pregnancy. More than 3 mice were studied for each group. Jasplakinolide (Jasp) used to inhibit F-actin depolymerization promotes F-actin polymerization in SMCs during implantation window and consequently compromises embryo implantation quality. Transcriptome analysis following Jasp treatment in mouse uterine SMCs reveals significant molecular changes associated with actin assembly. Tagln is involved in the regulation of the cell cytoskeleton and promotes the polymerization of G-actin to F-actin. Our results show that Tagln expression is gradually reduced in mouse uterine myometrium from day 1 to 4 of pregnancy. Furthermore, progesterone inhibits the expression of Tagln through the progesterone receptor. Using siRNA to knock down Tagln in day 3 SMCs, we found that phalloidin staining is decreased, which confirms the critical role of Tagln in F-actin polymerization. In conclusion, our data suggested that decreases in actin assembly in uterine smooth muscle during early pregnancy is critical to optimal embryo implantation. Tagln, a key molecule involved in actin assembly, regulates embryo implantation by controlling F-actin aggregation before implantation, suggesting moderate uterine contractility is conducive to embryo implantation. This study provides new insights into how the mouse uterus increases its flexibility to accommodate implanting embryos in the early stage of pregnancy.

Dataset Information

Unique uterine localization and regulation may differentiate LPA3 from other lysophospholipid receptors for its role in embryo implantation.

Objective

Design

Setting

Animal(s)

Intervention(s)

Main outcome measure(s)

Result(s)

Conclusion(s)

Publications

Unique uterine localization and regulation may differentiate LPA3 from other lysophospholipid receptors for its role in embryo implantation.

Similar Datasets

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